Biomedically, this project aims at classifying the TME, particularly tumor infiltrating lymphocytes (TIL). Since the presence of TILs and the composition of surface markers have been discussed controversially with regard to an impact on disease outcome, it is important to characterize TIL subtypes systematically to better understand immuno-phenotypes. This will be achieved on the example of pancreatic cancer, thereby categorizing disease promoting and rejecting immune cell types. Once characteristic immune cell populations with a responsive or non-responsive phenotype are identified, their existence will be confirmed in other tissue sections, obtained from healthy individuals or patients (i.e., diagnosed with Parkinson, Alzheimer, or other). The analysis tools you will develop in this PhD project will have great impact on answering clinically relevant questions and will contribute to the development of better diagnostics and therapies.